منابع مشابه
Extracellular Vesicle Flow Cytometry Analysis and Standardization
The term extracellular vesicles (EVs) describes membranous vesicles derived from cells, ranging in diameter from 30 to 1,000 nm with the majority thought to be in the region of 100-150 nm. Due to their small diameter and complex and variable composition, conventional techniques have struggled to accurately count and phenotype EVs. Currently, EV characterization using high-resolution flow cytome...
متن کامل-dependent Vesicle–Vesicle Fusion Events
A microneedle puncture of the fibroblast or sea urchin egg surface rapidly evokes a localized exocytotic reaction that may be required for the rapid resealing that follows this breach in plasma membrane integrity (Steinhardt, R.A,. G. Bi, and J.M. Alderton. 1994. Science (Wash. DC). 263:390–393). How this exocytotic reaction facilitates the resealing process is unknown. We found that starfish o...
متن کاملExtracellular vesicle sizing and enumeration by nanoparticle tracking analysis
Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should ...
متن کاملSteady state analysis of a vesicle bioreactor with mechanosensitive channels
An intermediate step between simple models of protocells having no membrane structure and the current, complex membrane organization of modern cells considers simple forms of membrane change that interact with the protocell dynamics in predictable ways. Here we analyse a kinetic model of a simple protocell system: a bioreactor vesicle involving mechanosensitive channels. This is a closed lipid ...
متن کاملAutomated analysis of secretory vesicle distribution at the ultrastructural level.
Neuroendocrine cells like chromaffin cells and PC-12 cells are established models for transport, docking and secretion of secretory vesicles. In micrographs, these vesicles are recognized by their electron dense core. The analysis of secretory vesicle distribution is usually performed manually, which is labour-intensive and subject to human bias and error. We have developed an algorithm to anal...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1979
ISSN: 0022-1554,1551-5044
DOI: 10.1177/27.11.512347